rabbit  (Alomone Labs)

Journal: bioRxiv

Article Title: Impaired excitability of fast-spiking neurons in a novel mouse model of KCNC1 epileptic encephalopathy

doi: 10.1101/2024.09.27.615463

Figure Lengend Snippet: A. Representative image of a cell being recorded in the outside-out nucleated macropatch configuration. B-C. Example family of traces of voltage-gated K + channel currents from a PV-IN from WT (B, black) and Kcnc1 -A421V/+ (C, green) mice. D. Average voltage-gated K + channel current density for WT ( n =13 macropatches, N =3 mice) and Kcnc1 -A421V/+ ( n =17, N =3 mice). E. Maximum K + channel current density per PV-IN macropatch in WT and Kcnc1 -A421V/+ mice. F. Averaged normalized plots of K + conductance relative to voltage command indicating voltage-dependence of activation curves for WT and Kcnc1 -A421V/+ mice. G. Average activation time constant for the voltage-gated K + channel currents relative to voltage command potential in both WT and Kcnc1 -A421V/+ mice. H. Representative images of individual cortical PV-INs from WT and Kcnc1 -A421V/+ mice stained for Kv3.1 (green). I. Group quantification of ratio of membrane to cytosolic Kv3.1 for WT ( n =27 cells, N =3 mice) and Kcnc1 -A421V/+ ( n =27 cells, N =3 mice). Data are shown as mean ± SEM or individual data points and significance was determined using either Repeated-measures Two-way ANOVA or unpaired t-test where ***P<0.001.

Article Snippet: The sections were then transferred into primary antibody solution containing rabbit Kv3.1b antibody (1:500; Alomone Labs APC-014) and mouse IgG1 parvalbumin antibody (EMD Millipore MAB1572) in blocking solution (1% NGS, 0.2% BSA, 0.3% Triton X-100 in PBS) and incubated at 4°C overnight.

Techniques: Activation Assay, Staining, Membrane